WTAP Primary Antibody
Item Information
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Size
Price
Description
The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in several transcript variants encoding three different isoforms.
Product Overview
Entrez GenelD
9589
Aliases
PNAS-132;hFL(2)D;KIAA0105
Clone#
6B6B8
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human WTAP (AA: 91-201) expressed in E. Coli.
Formulation
Purified antibody from tissue culture in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
J Gastroenterol. 2013 Nov;48(11):1271-82.
Cancer Sci. 2012 Dec;103(12):2102-9.
Cancer Sci. 2012 Dec;103(12):2102-9.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using WTAP mAb against human WTAP (AA: 91-201) recombinant protein. (Expected MW is 38.7 kDa)
Western Blot
Figure 3:Western blot analysis using WTAP mAb against HEK293 (1) and WTAP (AA: 91-201)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of Hela cells using WTAP mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using WTAP mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using WTAP mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.