WT1 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesGUD; AWT1; WAGR; WT33; NPHS4; WIT-2; EWS-WT1
ProductOrderW
Clone#5G11A5
Entrez GenelD7490
FormulationPurified antibody in PBS with 0.05% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human WT1 (AA: 314-479) expressed in E. Coli.
MW49.2kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
314-479
Catalog#: 30712
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Images
Western Blot
Figure 1: Western blot analysis using WT1 mAb against human WT1 (AA: 314-479) recombinant protein. (Expected MW is 47.6 kDa)
Figure 1: Western blot analysis using WT1 mAb against human WT1 (AA: 314-479) recombinant protein. (Expected MW is 47.6 kDa)
Western Blot
Figure 2: Western blot analysis using WT1 mAb against HEK293 (1) and WT1 (AA: 314-479)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using WT1 mAb against HEK293 (1) and WT1 (AA: 314-479)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 3: Flow cytometric analysis of Hela cells using WT1 mouse mAb (green) and negative control (red).
Figure 3: Flow cytometric analysis of Hela cells using WT1 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation site upstream of and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.

References (references)
References (references)1. Leuk Res. 2013 Oct;37(10):1341-9.
2. Pediatr Blood Cancer. 2013 Aug;60(8):1388-9.