WNT5A Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliaseshWNT5A
ProductOrderW
Clone#6F2
Entrez GenelD7474
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of WNT5A expressed in E. Coli.
MW42.3kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
WB (Western Blot)1/500 - 1/2000
Sequence
75-380
Catalog#: 20330
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Images
Western Blot
Figure 1: Western blot analysis using WNT5A mouse mAb against HEK293 (1) and WNT5A-hIgGFc transfected HEK293 cell lysate (2).
Figure 1: Western blot analysis using WNT5A mouse mAb against HEK293 (1) and WNT5A-hIgGFc transfected HEK293 cell lysate (2).
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer (A), thyroid cancer (B), lymph node (C) and brain (D) showing cytoplasmic and extracellular matrix localization using WNT5A mouse mAb with DAB staining.
Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer (A), thyroid cancer (B), lymph node (C) and brain (D) showing cytoplasmic and extracellular matrix localization using WNT5A mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 3: Confocal Immunofluorescence analysis of PC-12 cells using WNT5A mouse mAb (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.
Figure 3: Confocal Immunofluorescence analysis of PC-12 cells using WNT5A mouse mAb (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.
Product Overview
Description

WNT5A: wingless-type MMTV integration site family, member 5A. Entrez Protein: NP_003383. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98%, 98% and 87% amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction.

References (references)
References (references)1. Cancer Res. 2008 Jul 15;68(14):5785-94.
2. Proc Natl Acad Sci U S A. 2009 Mar 10;106(10):3919-24.