VIM Primary Antibody

Item Information

Catalog #

Size

Price

Description

This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients.

Product Overview

Entrez GenelD

7431

Clone#

3A1F2

Host / Isotype

Mouse / Mouse IgG2a

Immunogen

Purified recombinant fragment of human VIM (AA: 2-466) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200-1/1000

FCM (Flow Cytometry)

1/200-1/400

ELISA

1/10000

References

1.Pathol Res Pract. 2018 Sep;214(9):1376-1380. 2.Clin Cancer Res. 2018 Jan 15;24(2):420-432.

Product Image

ELISA

Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

WESTERN BLOT

Figure 2: Western blot analysis using VIM mAb against human VIM (AA: 2-466) recombinant protein. (Expected MW is 50 kDa)

WESTERN BLOT

Figure 3: Western blot analysis using VIM mAb against HEK293 (1) and VIM (AA: 2-466)-hIgGFc transfected HEK293 (2) cell lysate.

WESTERN BLOT

Figure 4: Western blot analysis using VIM mouse mAb against Jurkat (1), K562 (2), SK-N-SH (3), SH-SY5Y (4), Hela (5), NIH/3T3 (6), C6 (7), and RAW264.7 (8) cell lysate.

FLOW CYTOMETRY