VIL1 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesVIL; D2S1471
ProductOrderV
Clone#3E5G11
Entrez GenelD7429
FormulationPurified antibody in PBS with 0.05% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human VIL1 (AA: 1-209) expressed in E. Coli.
MW92.7kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
1-209
Catalog#: 30700
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Images
Western Blot
Figure 1: Western blot analysis using VIL1 mAb against human VIL1 (AA: 1-209) recombinant protein. (Expected MW is 49.4 kDa)
Figure 1: Western blot analysis using VIL1 mAb against human VIL1 (AA: 1-209) recombinant protein. (Expected MW is 49.4 kDa)
Western Blot
Figure 2: Western blot analysis using VIL1 mAb against HEK293 (1) and VIL1 (AA: 1-209)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using VIL1 mAb against HEK293 (1) and VIL1 (AA: 1-209)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using VIL1 mouse mAb against SW620 cell lysate.
Figure 3: Western blot analysis using VIL1 mouse mAb against SW620 cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of Hela cells using VIL1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4:Immunofluorescence analysis of Hela cells using VIL1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 5: Flow cytometric analysis of SW620 cells using VIL1 mouse mAb (green) and negative control (red).
Figure 5: Flow cytometric analysis of SW620 cells using VIL1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using VIL1 mouse mAb with DAB staining.
Figure 6: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using VIL1 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon.

References (references)
References (references)1. Cancer Sci. 2012 Aug;103(8):1493-501.
2. Cancer Biol Ther. 2011 Aug 1;12(3):181-90.