VIL1 Primary Antibody

Item Information

Catalog #

Size

Price

Description

This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon.

Product Overview

Entrez GenelD

7429

Aliases

VIL; D2S1471

Clone#

3E5G11

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human VIL1 (AA: 1-209) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide.

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1. Cancer Sci. 2012 Aug;103(8):1493-501.
2. Cancer Biol Ther. 2011 Aug 1;12(3):181-90.

Product Image

Western Blot

Figure 1: Western blot analysis using VIL1 mAb against human VIL1 (AA: 1-209) recombinant protein. (Expected MW is 49.4 kDa)

Western Blot

Figure 2: Western blot analysis using VIL1 mAb against HEK293 (1) and VIL1 (AA: 1-209)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 3: Western blot analysis using VIL1 mouse mAb against SW620 cell lysate.

Immunofluorescence analysis

Figure 4:Immunofluorescence analysis of Hela cells using VIL1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric