VAV1 Primary Antibody
Item Information
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Size
Price
Description
The protein encoded by this proto-oncogene is a member of the Dbl family of guanine nucleotide exchange factors (GEF) for the Rho family of GTP binding proteins. The protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. This particular GEF has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication.
Product Overview
Entrez GenelD
7409
Aliases
VAV
Clone#
2E11
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human VAV1 (AA: 121-324) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/250
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Acta Pharmacol Sin. 2011 Jan;32(1):99-107.
2.Cell Tissue Res. 2011 Jul;345(1):163-75.
2.Cell Tissue Res. 2011 Jul;345(1):163-75.
Product Image
Western Blot
Figure 1: Western blot analysis using VAV1 mAb against human VAV1 recombinant protein. (Expected MW is 49.3 kDa)
Western Blot
Figure 2: Western blot analysis using VAV1 mAb against HEK293 (1) and VAV1 (AA: 121-324)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using VAV1 mouse mAb against Jurkat (1) cell lysate.
Flow cytometric
Figure 4: Flow cytometric analysis of HeLa cells using VAV1 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.