UTF1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
Undifferentiated transcription factor-1 (UTF-1) is used as marker for the undifferentiated state of pluripotent stem cells.UTF1 is a chromatin-associated protein with core histone-like properties. UTF1 further acts as a transcriptional repressor and is required for proper differentiation of pluripotent cells.
Product Overview
Entrez GenelD
8433
Clone#
5B6
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human UTF1 (AA: 148-214) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Int J Exp Pathol. 2011 Oct;92(5):326-32. 2.Stem Cell Res. 2009 May;2(3):211-8.
Product Image
Western Blot
Figure 1: Western blot analysis using UTF1 mAb against human UTF1 recombinant protein. (Expected MW is 32.8 kDa)
Western Blot
Figure 2: Western blot analysis using UTF1 mAb against HEK293 (1) and UTF1 (AA: 148-214)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 3: Flow cytometric analysis of K562 cells using UTF1 mouse mAb (green) and negative control (purple).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.