UTF1 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
ProductOrderU
Clone#5B6
Entrez GenelD8433
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human UTF1 (AA: 148-214) expressed in E. Coli.
MW36.4kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
148-214
Catalog#: 30419
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Images
Western Blot
Figure 1: Western blot analysis using UTF1 mAb against human UTF1 recombinant protein. (Expected MW is 32.8 kDa)
Figure 1: Western blot analysis using UTF1 mAb against human UTF1 recombinant protein. (Expected MW is 32.8 kDa)
Western Blot
Figure 2: Western blot analysis using UTF1 mAb against HEK293 (1) and UTF1 (AA: 148-214)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using UTF1 mAb against HEK293 (1) and UTF1 (AA: 148-214)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 3: Flow cytometric analysis of K562 cells using UTF1 mouse mAb (green) and negative control (purple).
Figure 3: Flow cytometric analysis of K562 cells using UTF1 mouse mAb (green) and negative control (purple).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

Undifferentiated transcription factor-1 (UTF-1) is used as marker for the undifferentiated state of pluripotent stem cells.UTF1 is a chromatin-associated protein with core histone-like properties. UTF1 further acts as a transcriptional repressor and is required for proper differentiation of pluripotent cells.

References (references)
References (references)1.Int J Exp Pathol. 2011 Oct;92(5):326-32. 2.Stem Cell Res. 2009 May;2(3):211-8.