UPK3B

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesP35; UP3B; UPIIIB
Clone#3B2G1
Entrez GenelD105375355
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeMouse IgG1
ImmunogenPurified recombinant fragment of human UPK3B (AA: 30-180) expressed in E. Coli.
MW33.8kDa
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/50 - 1/200
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
30-180
Catalog#: 32293
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Images
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using UPK3B mAb against human UPK3B (AA: 30-180) recombinant protein. (Expected MW is 41.5 kDa)
Figure 2:Western blot analysis using UPK3B mAb against human UPK3B (AA: 30-180) recombinant protein. (Expected MW is 41.5 kDa)
Western Blot
Figure 3:Western blot analysis using UPK3B mAb against HEK293-6e (1) and UPK3B (AA: 30-180)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using UPK3B mAb against HEK293-6e (1) and UPK3B (AA: 30-180)-hIgGFc transfected HEK293-6e (2) cell lysate.
Immunohistochemical analysis
Figure 4:Immunofluorescence analysis of Hela cells using UPK3B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4:Immunofluorescence analysis of Hela cells using UPK3B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
Figure 5:Flow cytometric analysis of THP-1 cells using UPK3B mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of THP-1 cells using UPK3B mouse mAb (green) and negative control (red).
Product Overview
Description

UPK3B is a minor component of the apical plaques of mammalian urothelium that binds and dimerizes with uroplakin-1b (UPK1B; MIM 602380), one of the major conserved urothelium membrane proteins. The other major conserved integral membrane proteins of urothelial plaques are UPK1A (MIM 611557), UPK2 (MIM 611558), and UPK3A (MIM 611559) (Deng et al., 2002 [PubMed 12446744]).

References (references)
References (references)1.PLoS One. 2011;6(10):e25391.
2.Mol Phylogenet Evol. 2006 Nov;41(2):355-67.