UBB Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesUBC; UBA52; RPS27A
ProductOrderU
Clone#3C12
Entrez GenelD7314
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human UBB expressed in E. Coli.
MW26kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
full(1-299)
Catalog#: 30317
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Images
Western Blot
Figure 1: Western blot analysis using UBB mAb against human UBB (AA: 1-299) recombinant protein. (Expected MW is 26 kDa)
Figure 1: Western blot analysis using UBB mAb against human UBB (AA: 1-299) recombinant protein. (Expected MW is 26 kDa)
Western Blot
Figure 2: Western blot analysis using UBB mouse mAb against NIH/3T3 (1) and Hela (2) cell lysate.
Figure 2: Western blot analysis using UBB mouse mAb against NIH/3T3 (1) and Hela (2) cell lysate.
Flow cytometric
Figure 3: Flow cytometric analysis of Hela cells using UBB mouse mAb (green) and negative control (red).
Figure 3: Flow cytometric analysis of Hela cells using UBB mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome.

References (references)
References (references)1. Science. 2009 Aug 14;325(5942):834-40.
2. Biochem Soc Trans. 2009 Oct;37(Pt 5):937-53.