Mouse Monoclonal Antibody to TUBB1
Item Information
Catalog #
Size
Price
Description
This gene encodes a member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is specifically expressed in platelets and megakaryocytes and may be involved in proplatelet production and platelet release. A mutations in this gene is associated with autosomal dominant macrothrombocytopenia. Two pseudogenes of this gene are found on chromosome Y.
Product Overview
Entrez GenelD
203068
Aliases
M40; TUBB1; TUBB5; CDCBM6; CSCSC1; OK/SW-cl.56
Clone#
4D4G6
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human TUBB1 (AA: 1-444) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Int J Mol Sci. 2020 Feb 18;21(4):1385.2,Biochem Biophys Res Commun. 2016 Aug 5;476(4):273-279.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using TUBB1 mAb against human TUBB1 (AA: 1-444) recombinant protein. (Expected MW is 52.6 kDa)
Western Blot
Figure 3:Western blot analysis using TUBB1 mAb against HEK293-6e (1) and human TUBB1 (AA: 1-444)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using TUBB1 mouse mAb against K562 (1), HepG2 (2), A431 (3), Jurkat (4), Hela (5), NIH/3T3 (6), and COS-7 (7) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of Hela cells using TUBB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
Figure 6:Flow cytometric analysis of Hela cells using TUBB1 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 7:Flow cytometric analysis of HepG2 cells using TUBB1 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 8:Flow cytometric analysis of HL-60 cells using TUBB1 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 9:Flow cytometric analysis of Jurkat cells using TUBB1 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 10:Flow cytometric analysis of THP-1 cells using TUBB1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 11:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using TUBB1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 12:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using TUBB1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 13:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using TUBB1 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.