TUBB1 Primary Antibody
|Formulation||Purified antibody in PBS with 0.05% sodium azide|
|Immunogen||Purified recombinant fragment of human TUBB1 (AA: 33-166) expressed in E. Coli.|
|Shipping Information||This product will ship in a box containing blue ice at a temperature of 4°C. Learn More|
|Species Reactivity||Human, Mouse, Monkey, Rat|
|ICC (Immunocytochemistry)||1/200 - 1/1000|
|IHC_P(Immunohistochemistry)||1/200 - 1/1000|
|FCM (Flow Cytometry)||1/200 - 1/400|
|WB (Western Blot)||1/500 - 1/2000|
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 2:Western blot analysis using TUBB1 mAb against human TUBB1 (AA: 33-166) recombinant protein. (Expected MW is 40.5 kDa)
Figure 3:Western blot analysis using TUBB1 mAb against HEK293 (1) and TUBB1 (AA: 33-166)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Western blot analysis using TUBB1 mouse mAb against K562 (1), HepG2 (2), A431 (3), Jurkat (4), Hela (5), NIH/3T3 (6), Cos7 (7) and PC12 (8) cell lysate.
Figure 5:Immunofluorescence analysis of Hela cells using TUBB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 6:Flow cytometric analysis of A431 cells using TUBB1 mouse mAb (green) and negative control (red).
Figure 7:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using TUBB1 mouse mAb with DAB staining.
Figure 8:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using TUBB1 mouse mAb with DAB staining.
|Description||This gene encodes a member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is specifically expressed in platelets and megakaryocytes and may be involved in proplatelet production and platelet release. A mutations in this gene is associated with autosomal dominant macrothrombocytopenia. Two pseudogenes of this gene are found on chromosome Y.|
|References (references)||Cancer Res. 2012 Sep 15;72(18):4744-52. |
Cytoskeleton (Hoboken). 2011 Mar;68(3):175-87.