TUBA8 Primary Antibody

Item Information

Catalog #

Size

Price

Description

This gene encodes a member of the alpha tubulin protein family. Alpha tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Mutations in this gene are associated with polymicrogyria and optic nerve hypoplasia. Alternate splicing results in multiple transcript variants.Â

Product Overview

Entrez GenelD

51807

Aliases

TUBAL2

Clone#

2D6

Host / Isotype

Mouse / IgG2b

Species Reactivity

Human, Rat

Immunogen

Purified recombinant fragment of human TUBA8 expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1. Am J Hum Genet. 2009 Nov;85(5):737-44.
2. Am J Hum Genet. 2009 Nov;85(5):628-42.

Product Image

Western Blot

Figure 1: Western blot analysis using TUBA8 mAb against human TUBA8 (AA: 294-449) recombinant protein. (Expected MW is 50 kDa)

Western Blot

Figure 2: Western blot analysis using TUBA8 mouse mAb against rat heart (1) tissue lysate.

Immunohistochemical analysis

Figure 3: Immunohistochemical analysis of paraffin-embedded medulla oblongata tissues using TUBA8 mouse mAb with DAB staining.

Immunohistochemical analysis

Figure 4: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using TUBA8 mouse mAb with DAB staining.

Immunofluorescence analysis

Figure 5: Immunofluorescence analysis of Hela cells using TUBA8 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow cytometric