TUBA8 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesTUBAL2
ProductOrderT
Clone#2D6
Entrez GenelD51807
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG2b
ImmunogenPurified recombinant fragment of human TUBA8 expressed in E. Coli.
MW50kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman, Rat
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
294-449
Catalog#: 30301
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Images
Western Blot
Figure 1: Western blot analysis using TUBA8 mAb against human TUBA8 (AA: 294-449) recombinant protein. (Expected MW is 50 kDa)
Figure 1: Western blot analysis using TUBA8 mAb against human TUBA8 (AA: 294-449) recombinant protein. (Expected MW is 50 kDa)
Western Blot
Figure 2: Western blot analysis using TUBA8 mouse mAb against rat heart (1) tissue lysate.
Figure 2: Western blot analysis using TUBA8 mouse mAb against rat heart (1) tissue lysate.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded medulla oblongata tissues using TUBA8 mouse mAb with DAB staining.
Figure 3: Immunohistochemical analysis of paraffin-embedded medulla oblongata tissues using TUBA8 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using TUBA8 mouse mAb with DAB staining.
Figure 4: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using TUBA8 mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 5: Immunofluorescence analysis of Hela cells using TUBA8 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 5: Immunofluorescence analysis of Hela cells using TUBA8 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric
Figure 6: Flow cytometric analysis of NIH/3T3 cells using TUBA8 mouse mAb (green) and negative control (red).
Figure 6: Flow cytometric analysis of NIH/3T3 cells using TUBA8 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

This gene encodes a member of the alpha tubulin protein family. Alpha tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Mutations in this gene are associated with polymicrogyria and optic nerve hypoplasia. Alternate splicing results in multiple transcript variants. 

References (references)
References (references)1. Am J Hum Genet. 2009 Nov;85(5):737-44.
2. Am J Hum Genet. 2009 Nov;85(5):628-42.