Mouse Monoclonal Antibody to TSG101
Item Information
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Price
Description
The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression.
Product Overview
Entrez GenelD
7251
Aliases
TSG10; VPS23
Clone#
8E10D10
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human TSG101 (AA: 167-374) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,J Biol Chem. 2019 Nov 1;294(44):16266-16282. 2,Int J Mol Sci. 2019 Feb 12;20(3):774.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using TSG101 mAb against human TSG101 (AA: 167-374) recombinant protein. (Expected MW is 26.3 kDa)
Western Blot
Figure 3:Western blot analysis using TSG101 mAb against HEK293-6e (1) and human TSG101 (AA: 167-374)-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
Figure 4:Flow cytometric analysis of THP-1 cells using TSG101 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using TSG101 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using TSG101 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.