Trim5a Primary Antibody
Item Information
Catalog #
Size
Price
Description
TRIM5-alpha is a protein that is found in the cells of many mammals and fends of various retrovirus infections. It protects monkeys from infection with HIV-1, and humans from infection with some other viruses. If a retrovirus has entered a cell, it needs to shed its capsid in order to reversely transcribe its genes, so that they can be expressed by the host cell. It is believed that TRIM5 alpha, which is present in the cytoplasm, somehow recognizes the capsid and blocks its shedding, thereby stopping the virus in its tracks. It thus represents an intracellular defense completely separate from the rest of the body's immune system.
Product Overview
Entrez GenelD
85363
Aliases
RNF88; TRIM5alpha
Clone#
3B11H2
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human trim5 alpha expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. Stremlau, M. Nature 2004.427:848-53.
2. Song, B. J Virol. 2005.79(7):3930-7.
2. Song, B. J Virol. 2005.79(7):3930-7.
Product Image
Western Blot
Figure 1: Western blot analysis using Trim5? mouse mAb against human breast carcinoma tissue lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded human metastatic adenocarcinoma(A) and stomach adenocarcinoma (B), showing cytoplasmic localization using Trim5? mouse mAb with AEC staining (A) and DAB staining(B).
Western Blot
Figure 3: Western blot analysis using Trim5? mouse monoclonal antiobdy against truncated Trim5?recombinant protein.
For Research Use Only. Not for use in diagnostic procedures.