Mouse Monoclonal Antibody to TRBC1
Item Information
Catalog #
Size
Price
Description
TRBC1 (T Cell Receptor Beta Constant 1) is a Protein Coding gene. Among its related pathways are Translocation of ZAP-70 to Immunological synapse and Innate Immune System. An important paralog of this gene is TRBC2.
Product Overview
Entrez GenelD
28639
Aliases
TCRB; TCRBC1; BV05S1J2.2
Clone#
5D9F1
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human TRBC1 (AA: 1-149) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/100 - 1/500
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Proc Natl Acad Sci U S A. 1985 Aug;82(15):5068-72. 2.Virchows Arch. 2005 Jan;446(1):15-20.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/7d04371c4973582128090633273ba5a95cfbe159895193881765541e044f0707.jpg?v=1666706089)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using TRBC1 mAb against human TRBC1 (AA: 1-149) recombinant protein. (Expected MW is 42.8 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/300f9a62c90f3a6f46a66da6c245749b0f3e00134275ba6591ae8b2dfa688897.jpg?v=1666706089)
Figure 2:Western blot analysis using TRBC1 mAb against human TRBC1 (AA: 1-149) recombinant protein. (Expected MW is 42.8 kDa)
Western Blot
![Figure 3:Western blot analysis using TRBC1 mAb against HEK293-6e (1) and TRBC1 (AA: 1-149)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/510115983e75d6fdfa776b1ad69d9d807cbd9576abdcab23c57ec8768fd97a45.jpg?v=1666706089)
Figure 3:Western blot analysis using TRBC1 mAb against HEK293-6e (1) and TRBC1 (AA: 1-149)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
![Figure 4:Western blot analysis using TRBC1 mouse mAb against HUVEC (1), Jurkat (2), Hela (3), HUVE-12 (4), A549 (5), C6 (6), Raji (7), and T47D (8) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/40ef129e4303a7683aca35e6f84fca4ce0386437c09ff4a51823f8281ed715be.jpg?v=1666706089)
Figure 4:Western blot analysis using TRBC1 mouse mAb against HUVEC (1), Jurkat (2), Hela (3), HUVE-12 (4), A549 (5), C6 (6), Raji (7), and T47D (8) cell lysate.
Immunofluorescence analysis
![Figure 5:Immunofluorescence analysis of Hela cells using TRBC1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/2b2bfc66772ae640bc67dc1ccae5be2eb1e2eb554000aa03df505f891fc05f28.jpg?v=1666706089)
Figure 5:Immunofluorescence analysis of Hela cells using TRBC1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
![Figure 6:Flow cytometric analysis of Jurkat cells using TRBC1 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/d8c744f2b4a2e31d745e68ed024c1082079224fc555dab6b85e08dfba88c6349.jpg?v=1666706089)
Figure 6:Flow cytometric analysis of Jurkat cells using TRBC1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
![Figure 7:Immunohistochemical analysis of paraffin-embedded testis tissues using TRBC1 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/0d96634bbadea001e081d69aeaa38551c04e181b148fb84dec92e6a3a8e24ca7.jpg?v=1666706090)
Figure 7:Immunohistochemical analysis of paraffin-embedded testis tissues using TRBC1 mouse mAb with DAB staining.
Immunohistochemical analysis
![Figure 8:Immunohistochemical analysis of paraffin-embedded cerebellum tissues using TRBC1 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/ff97ce9afcdccff8e1c4f6e33194e93d98e853a5443c4f1bbfea5ccd24be1bb2.jpg?v=1666706090)
Figure 8:Immunohistochemical analysis of paraffin-embedded cerebellum tissues using TRBC1 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.