TNFSF13B Primary Antibody

Item Information

Catalog #

Size

Price

Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. Alternatively spliced transcript variants encoding distinct isoforms have been identified.

Product Overview

Entrez GenelD

10673

Aliases

DTL; BAFF; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNLG7A; TNFSF20

Clone#

6C3A2

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human TNFSF13B (AA: 116-278) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/100 - 1/500

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.PLoS One. 2015 Nov 23;10(11):e0143393.
2.Biomed Res Int. 2015;2015:792187.

Product Image

Elisa

Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using TNFSF13B mAb against human TNFSF13B (AA: 116-278) recombinant protein. (Expected MW is 44.1 kDa)

Western Blot

Figure 3:Western blot analysis using TNFSF13B mAb against HEK293 (1) and TNFSF13B (AA: 116-278)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using TNFSF13B mouse mAb against SK-N-SH (1) and MOLT4 (2) cell lysate.

Immunofluorescence analysis

Figure 5:Immunofluorescence analysis of SMMC-7721 cells using TNFSF13B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric