TNFSF13B Primary Antibody
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Description
The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. Alternatively spliced transcript variants encoding distinct isoforms have been identified.
Product Overview
Immunogen
Purified recombinant fragment of human TNFSF13B (AA: 116-278) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using TNFSF13B mAb against human TNFSF13B (AA: 116-278) recombinant protein. (Expected MW is 44.1 kDa)
Western Blot
Figure 3:Western blot analysis using TNFSF13B mAb against HEK293 (1) and TNFSF13B (AA: 116-278)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using TNFSF13B mouse mAb against SK-N-SH (1) and MOLT4 (2) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of SMMC-7721 cells using TNFSF13B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 6:Flow cytometric analysis of HeLa cells using TNFSF13B mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.