TNFSF11 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.
Product Overview
Entrez GenelD
8600
Aliases
CD254; ODF; OPGL; sOdf; OPTB2; RANKL; TNLG6B; TRANCE; hRANKL2
Clone#
3H8A7
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human TNFSF11 (AA: 74-308) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Breast Cancer Res. 2015 Feb 21;17:24.
2.Immunobiology. 2015 May;220(5):692-700.
2.Immunobiology. 2015 May;220(5):692-700.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using TNFSF11 mAb against human TNFSF11 (AA: 74-308) recombinant protein. (Expected MW is 52.6 kDa)
Western Blot
Figure 3:Western blot analysis using TNFSF11 mAb against HEK293 (1) and TNFSF11 (AA: 74-308)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using TNFSF11 mouse mAb against U937 (1), HL-60 (2), Raji (3), and Ramos (4) cell lysate.
Flow cytometric
Figure 5:Flow cytometric analysis of Hela cells using TNFSF11 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using TNFSF11 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.