TIM3 Primary Antibody

Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance.
Product Overview
Entrez GenelD
84868
Aliases
HAVCR2; CD366; KIM-3; TIMD3; Tim-3; TIMD-3; HAVcr-2
Clone#
7C8A7
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human TIM3 (AA: extra 22-202) expressed in HEK293 cells.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Tumour Biol. 2016 Jun;37(6):8209-18.
2.Int Immunopharmacol. 2015 Dec;29(2):635-641.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using TIM3 mAb against human TIM3 (AA: extra 22-202) recombinant protein. (Expected MW is 50 kDa)
Figure 2:Western blot analysis using TIM3 mAb against human TIM3 (AA: extra 22-202) recombinant protein. (Expected MW is 50 kDa)
Immunofluorescence analysis
Figure 3:Immunofluorescence analysis of Hela cells using TIM3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 3:Immunofluorescence analysis of Hela cells using TIM3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 4:Flow cytometric analysis of Jurkat cells using TIM3 mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of Jurkat cells using TIM3 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.