TH Primary Antibody

Item Information

Catalog #

Size

Price

Description

The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Product Overview

Entrez GenelD

7054

Aliases

TYH; DYT14; DYT5b

Clone#

1B8D2

Host / Isotype

Mouse / IgG1

Species Reactivity

Human, Rat

Immunogen

Purified recombinant fragment of human TH (AA: 44-208) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Neurosci Lett. 2014 Jan 24;559:39-43.
2.Biochem Biophys Res Commun. 2011 Nov 4;414(4):712-8.

Product Image

Elisa

Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using TH mAb against human TH (AA: 44-208) recombinant protein. (Expected MW is 44 kDa)

Western Blot

Figure 3:Western blot analysis using TH mAb against HEK293 (1) and TH (AA: 44-208)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using TH mouse mAb against SH-SY5Y (1) and PC-12 (2) cell lysate.

Immunofluorescence analysis

Figure 5:Immunofluorescence analysis of Hela cells using TH mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric