TFRC Primary Antibody
Item Information
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Description
Transferrin receptor is a carrier protein for transferrin. It is needed for the import of iron into the cell and is regulated in response to intracellular iron concentration. Low iron concentrations promote increased levels of transferrin receptor, to increase iron intake into the cell. Thus, transferrin receptor maintains cellular iron homeostasis. Expression of human TFR1, but not human TFR2, in hamster cell lines markedly enhanced the infection of viruses pseudotyped with the glycoprotein of Machupo, Guanarito, and Junin viruses, but not with those of Lassa or lymphocytic choriomeningitis viruses. An anti-TFR1 antibody efficiently inhibited the replication of Machupo, Guanarito, Junin, and Sabia viruses, but not that of Lassa virus.TFR1 is a cellular receptor for New World hemorrhagic fever arenaviruses.
Product Overview
Entrez GenelD
7037
Aliases
T9; TR; TFR; p90; CD71; TFR1; TRFR
Clone#
1A1B2
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Mouse, Monkey, Rat
Immunogen
Purified recombinant fragment of human TFRC (AA: 608-727) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ELISA
1/10000
References
1. Folia Histochem Cytobiol. 2012 Jul 5;50(2):304-11.
2. J Biol Chem. 2011 Oct 14;286(41):35708-15.
2. J Biol Chem. 2011 Oct 14;286(41):35708-15.
Product Image
Western Blot
Figure 1: Western blot analysis using TFRC mAb against human TFRC recombinant protein. (Expected MW is 39.7 kDa)
Western Blot
Figure 2: Western blot analysis using TFRC mAb against HEK293 (1) and TFRC (AA: 608-727)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using TFRC mouse mAb against Jurkat (1), Hela (2), K562 (3), Cos7 (4), MCF-7 (5), PC-12 (6), NIH/3T3 (7), HEK293 (8), RAJI (9) cell lysate.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.