Mouse Monoclonal Antibody to TDP43
Item Information
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Description
HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq, Jul 2008]
Product Overview
Entrez GenelD
23435
Aliases
ALS10; TDP-43
Clone#
6G2D8
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human TDP43 (AA: free peptide) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Adv Exp Med Biol. 2021;1281:201-217.2,Science. 2021 Feb 5;371(6529):eabb4309.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using TDP43 mouse mAb against Hela (1), HEK293 (2), MCF-7 (3), and A549 (4) cell lysate.
Flow cytometric analysis
Figure 3:Flow cytometric analysis of A431 cells using TDP43 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 4:Flow cytometric analysis of Hela cells using TDP43 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 5:Flow cytometric analysis of HepG2 cells using TDP43 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded baldder cancer tissues using TDP43 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded liver cancer tissues using TDP43 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using TDP43 mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 9:Immunofluorescence analysis of Hela cells using TDP43 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
For Research Use Only. Not for use in diagnostic procedures.