TARDBP Primary Antibody
|Formulation||Purified antibody in PBS with 0.05% sodium azide|
|Immunogen||Purified recombinant fragment of human TARDBP (AA: 126-260) expressed in E. Coli.|
|Shipping Information||This product will ship in a box containing blue ice at a temperature of 4°C. Learn More|
|FCM (Flow Cytometry)||1/200 - 1/400|
|WB (Western Blot)||1/500 - 1/2000|
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 2:Western blot analysis using TARDBP mAb against human TARDBP (AA: 126-260) recombinant protein. (Expected MW is 41.5 kDa)
Figure 3:Western blot analysis using TARDBP mAb against HEK293 (1) and TARDBP (AA: 126-260)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Flow cytometric analysis of Hela cells using TARDBP mouse mAb (green) and negative control (red).
|Description||HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. |
|References (references)||1.Exp Cell Res. 2013 Aug 1;319(13):1998-2005. |
2.PLoS One. 2013 May 30;8(5):e64002.