SYK Primary Antibody

Item Information

Catalog #

Size

Price

Description

This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Product Overview

Entrez GenelD

6850

Aliases

p72-Syk

Clone#

2G11E6

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human SYK (AA: 217-356) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/50 - 1/250

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Clin Cancer Res. 2015 Jun 1;21(11):2538-45.
2.PLoS One. 2014 Feb 11;9(2):e87610.

Product Image

Elisa

Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using SYK mAb against human SYK (AA: 217-356) recombinant protein. (Expected MW is 41.4 kDa)

Western Blot

Figure 3:Western blot analysis using SYK mAb against HEK293 (1) and SYK (AA: 217-356)-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis

Figure 4:Immunofluorescence analysis of Hela cells using SYK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric