STAT3 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesAPRF; HIES; FLJ20882; MGC16063; STAT3
ProductOrderS
Clone#3B5
Entrez GenelD6774
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human STAT3 expressed in E. Coli.
MW88kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman, Mouse, Monkey
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
WB (Western Blot)1/500 - 1/2000
Sequence
20-240
Catalog#: 30122
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Images
Western Blot
Figure 1: Western blot analysis using STAT3 mouse mAb against Hela (1),NIH/3T3 (2), Jurkat (3), PC-12 (4) and COS7 (5) cell lysate.
Figure 1: Western blot analysis using STAT3 mouse mAb against Hela (1),NIH/3T3 (2), Jurkat (3), PC-12 (4) and COS7 (5) cell lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded mammary cancer tissues (left) and lung cancer tissues (right) using STAT3 mouse mAb with DAB staining.
Figure 2: Immunohistochemical analysis of paraffin-embedded mammary cancer tissues (left) and lung cancer tissues (right) using STAT3 mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of Hela cells using STAT3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 3: Immunofluorescence analysis of Hela cells using STAT3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Product Overview
Description

The Stat3 transcription factor is an important signaling molecule for many cytokines and growth-factor receptors and is required for murine fetal development . Stat3 is constitutively activated in a number of human tumors and possesses oncogenic potential and anti-apoptotic activities. Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation and DNA binding. Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways. Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3a (86 kDa) and Stat3

References (references)
References (references)1. J Mol Graph Model. 2009 Nov;28(4):347-56.
2. Bone. 2010 Feb;46(2):524-33.