SPP1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene.
Product Overview
Entrez GenelD
6696
Aliases
OPN; BNSP; BSPI; ETA-1
Clone#
7C5H12
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human SPP1 (AA: 167-314) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1.Blood. 2012 May 31;119(22):5215-20.
2.J Cancer Res Ther. 2011 Apr-Jun;7(2):138-42.
2.J Cancer Res Ther. 2011 Apr-Jun;7(2):138-42.
Product Image
Western Blot
Figure 1: Western blot analysis using SPP1 mAb against human SPP1 recombinant protein. (Expected MW is 42.6 kDa)
Western Blot
Figure 2: Western blot analysis using SPP1 mAb against HEK293 (1) and SPP1 (AA: 167-314)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using SPP1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using SPP1 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.