SP10 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a testis-specific, differentiation antigen, acrosomal vesicle protein 1, that arises within the acrosomal vesicle during spermatogenesis, and is associated with the acrosomal membranes and matrix of mature sperm. The acrosomal vesicle protein 1 may be involved in sperm-zona binding or penetration. Alternatively spliced transcript variants have been described.
Product Overview
Entrez GenelD
56
Aliases
ACRV1,SP-10; SPACA2; D11S4365
Clone#
2D10D5
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human SP10 (AA: 22-265) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200-1/400
ELISA
1/10000
References
1,Biol Reprod. 1994 Dec;51(6):1222-31.2,Genomics. 1993 Nov;18(2):446-9.
Product Image
ELISA
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
WESTERN BLOT
Figure 2: Western blot analysis using SP10 mAb against human SP10 (AA: 22-265) recombinant protein. (Expected MW is 28.8 kDa)
WESTERN BLOT
Figure 3: Western blot analysis using SP10 mAb against HEK293-6e (1) and SP10 (AA: 22-265)-hIgGFc transfected HEK293-6e (2) cell lysate.
FLOW CYTOMETRY
Figure 4: Flow cytometric analysis of Hela cells using SP10 mouse mAb (green) and negative control (red).
FLOW CYTOMETRY
Figure 5: Flow cytometric analysis of SK-OV-3 cells using SP10 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.