SMN1 Primary Antibody

Item Information

Catalog #

Size

Price

Description

This gene is part of a 500 kb inverted duplication on chromosome 5q13. This duplicated region contains at least four genes and repetitive elements which make it prone to rearrangements and deletions. The repetitiveness and complexity of the sequence have also caused difficulty in determining the organization of this genomic region. The telomeric and centromeric copies of this gene are nearly identical and encode the same protein.

Product Overview

Entrez GenelD

6606

Aliases

SMA; SMN; SMA1; SMA2; SMA3; SMA4; SMA@; SMN2; SMNT; BCD541; T-BCD541

Clone#

5H1

Host / Isotype

Mouse / IgG1

Species Reactivity

Human, Monkey

Immunogen

Purified recombinant fragment of human SMN1 expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1. J Med Genet. 2009 Sep;46(9):641-4.
2. RNA. 2009 Apr;15(4):515-23.

Product Image

Western Blot

Figure 1: Western blot analysis using SMN1 mouse mAb against RAJI (1), Cos7 (2), Jurkat (3), K562 (4), Hela (5) and HepG2 (6) cell lysate.

Immunohistochemical analysis

Figure 2: Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and testis tissues (right) using SMN1 mouse mAb with DAB staining.

Immunohistochemical analysis

Figure 3: Immunohistochemical analysis of paraffin-embedded stomach cancer tissues (left) and brain tumor (right) using SMN1 mouse mAb with DAB staining.

Immunofluorescence analysis

Figure 4: Immunofluorescence analysis of HepG2 cells using SMN1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

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