SMCP Primary Antibody

Item Information
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Price
Description

Sperm mitochondria differ in morphology and subcellular localization from those of somatic cells. They are elongated, flattened, and arranged circumferentially to form a helical coiled sheath in the midpiece of the sperm flagellum. The protein encoded by this gene localizes to the capsule associated with the mitochondrial outer membranes and is thought to function in the organization and stabilization of the helical structure of the sperm's mitochondrial sheath.

Product Overview
Entrez GenelD
4184
Aliases
MCS; MCSP; HSMCSGEN1
Clone#
5C10D8
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human SMCP (AA: FULL(1-116)) expressed in E. Coli.
Formulation
Purified antibody from tissue culture in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. Biol Reprod. 1999 Aug;61(2):428-35.
2. Mol Cell Biol. 2002 May;22(9):3046-52.
Product Image
Western Blot
Figure 1: Western blot analysis using SMCP mAb against human SMCP (AA: FULL(1-116)) recombinant protein. (Expected MW is 38.3 kDa)
Figure 1: Western blot analysis using SMCP mAb against human SMCP (AA: FULL(1-116)) recombinant protein. (Expected MW is 38.3 kDa)
Western Blot
Figure 2: Western blot analysis using SMCP mAb against HEK293 (1) and SMCP (AA: FULL(1-116))-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using SMCP mAb against HEK293 (1) and SMCP (AA: FULL(1-116))-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 4: Immunofluorescence analysis of Hela cells using SMCP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)
Figure 4: Immunofluorescence analysis of Hela cells using SMCP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.