SMCP Primary Antibody

Item Information

Catalog #

Size

Price

Description

Sperm mitochondria differ in morphology and subcellular localization from those of somatic cells. They are elongated, flattened, and arranged circumferentially to form a helical coiled sheath in the midpiece of the sperm flagellum. The protein encoded by this gene localizes to the capsule associated with the mitochondrial outer membranes and is thought to function in the organization and stabilization of the helical structure of the sperm's mitochondrial sheath.

Product Overview

Entrez GenelD

4184

Aliases

MCS; MCSP; HSMCSGEN1

Clone#

5C10D8

Host / Isotype

Mouse / IgG2b

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human SMCP (AA: FULL(1-116)) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1. Biol Reprod. 1999 Aug;61(2):428-35.
2. Mol Cell Biol. 2002 May;22(9):3046-52.

Product Image

Western Blot

Figure 1: Western blot analysis using SMCP mAb against human SMCP (AA: FULL(1-116)) recombinant protein. (Expected MW is 38.3 kDa)

Western Blot

Figure 2: Western blot analysis using SMCP mAb against HEK293 (1) and SMCP (AA: FULL(1-116))-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis

Figure 4: Immunofluorescence analysis of Hela cells using SMCP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)

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