SMAD1 Primary Antibody

Item Information
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Size
Price
Description
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.
Product Overview
Entrez GenelD
4086
Aliases
BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Clone#
7G11G7
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Monkey
Immunogen
Purified recombinant fragment of human SMAD1 (AA: 1-110) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ELISA
1/10000
References
1.Histol Histopathol. 2011 Apr;26(4):531-41.
2.Blood. 2011 Jun 16;117(24):6489-97.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using SMAD1 mAb against human SMAD1 (AA: 1-110) recombinant protein. (Expected MW is 38.5 kDa)
Figure 2:Western blot analysis using SMAD1 mAb against human SMAD1 (AA: 1-110) recombinant protein. (Expected MW is 38.5 kDa)
Western Blot
Figure 3:Western blot analysis using SMAD1 mAb against HEK293 (1) and SMAD1 (AA: 1-110)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using SMAD1 mAb against HEK293 (1) and SMAD1 (AA: 1-110)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using SMAD1 mouse mAb against COS7 (1), HUVEC (2), and C2C12 (3) cell lysate.
Figure 4:Western blot analysis using SMAD1 mouse mAb against COS7 (1), HUVEC (2), and C2C12 (3) cell lysate.
For Research Use Only. Not for use in diagnostic procedures.