SKP1 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesOCP2; p19A; EMC19; SKP1A; OCP-II; TCEB1L
ProductOrderS
Clone#1H9
Entrez GenelD6500
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human SKP1 expressed in E. Coli.
MW19kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
FULL(1-160)
Catalog#: 30316
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Images
Western Blot
Figure 1: Western blot analysis using SKP1 mAb against human SKP1 (AA: 1-160) recombinant protein. (Expected MW is 50 kDa)
Figure 1: Western blot analysis using SKP1 mAb against human SKP1 (AA: 1-160) recombinant protein. (Expected MW is 50 kDa)
Western Blot
Figure 2: Western blot analysis using SKP1 mouse mAb against Hela (1), RAJI (2), Jurkat (3), MCF-7 (4), HepG2 (5), PC-12 (6) and Cos7 (7) cell lysate.
Figure 2: Western blot analysis using SKP1 mouse mAb against Hela (1), RAJI (2), Jurkat (3), MCF-7 (4), HepG2 (5), PC-12 (6) and Cos7 (7) cell lysate.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using SKP1 mouse mAb with DAB staining.
Figure 3: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using SKP1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using SKP1 mouse mAb with DAB staining.
Figure 4: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using SKP1 mouse mAb with DAB staining.
Flow cytometric
Figure 5: Flow cytometric analysis of Hela cells using SKP1 mouse mAb (green) and negative control (red).
Figure 5: Flow cytometric analysis of Hela cells using SKP1 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

This gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7.

References (references)
References (references)1.J Biol Chem. 2008 Nov 7;283(45):30796-803.
2.Cell. 2009 Jul 23;138(2):389-403.