SIRT4 Primary Antibody
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family.
Purified antibody in PBS with 0.05% sodium azide
4°C; -20°C for long term storage
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 2:Western blot analysis using SIRT4 mAb against human SIRT4 (AA: 215-314) recombinant protein. (Expected MW is 37 kDa)
Figure 3:Western blot analysis using SIRT4 mAb against HEK293 (1) and SIRT4 (AA: 215-314)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Immunofluorescence analysis of HeLa cells using SIRT4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Flow cytometric analysis of HeLa cells using SIRT4 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.