SIRT3 Primary Antibody
Item Information
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Size
Price
Description
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene.
Product Overview
Entrez GenelD
23410
Aliases
SIR2L3
Clone#
6B2A1
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human SIRT3 (AA: 155-290) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Biomed Res Int. 2014;2014:871263.
2.Med Oncol. 2014 Aug;31(8):103.
2.Med Oncol. 2014 Aug;31(8):103.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using SIRT3 mAb against human SIRT3 (AA: 155-290) recombinant protein. (Expected MW is 41.3 kDa)
Western Blot
Figure 3:Western blot analysis using SIRT3 mAb against HEK293 (1) and SIRT3 (AA: 155-290)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 5:Flow cytometric analysis of HeLa cells using SIRT3 mouse mAb (green) and negative control (red).
Western Blot
Figure 7:Western blot analysis using SIRT3 mouse mAb against PANC-1 (1) cell lysate.
For Research Use Only. Not for use in diagnostic procedures.