SHC1 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesSHC; SHCA
ProductOrderS
Clone#2F7C7
Entrez GenelD6464
FormulationPurified antibody in PBS with 0.05% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human SHC1 (AA: 385-495) expressed in E. Coli.
MW62.8kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman, Mouse
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
385-495
Catalog#: 30689
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Images
Western Blot
Figure 1: Western blot analysis using SHC1 mAb against human SHC1 (AA: 385-495) recombinant protein. (Expected MW is 37.3 kDa)
Figure 1: Western blot analysis using SHC1 mAb against human SHC1 (AA: 385-495) recombinant protein. (Expected MW is 37.3 kDa)
Western Blot
Figure 2: Western blot analysis using SHC1 mAb against HEK293 (1) and SHC1 (AA: 385-495)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using SHC1 mAb against HEK293 (1) and SHC1 (AA: 385-495)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using SHC1 mouse mAb against NIH/3T3 cell lysate.
Figure 3: Western blot analysis using SHC1 mouse mAb against NIH/3T3 cell lysate.
Immunofluorescence analysis
Figure 4: Immunofluorescence analysis of A431 cells using SHC1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4: Immunofluorescence analysis of A431 cells using SHC1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 5: Flow cytometric analysis of NIH/3T3 cells using SHC1 mouse mAb (green) and negative control (red).
Figure 5: Flow cytometric analysis of NIH/3T3 cells using SHC1 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene.

References (references)
References (references)1. Am J Physiol Heart Circ Physiol. 2012 Feb 1;302(3):H724-32.
2. Clin Cardiol. 2010 Sep;33(9):548-52.