SHC1 Primary Antibody
| Item Information | ||
|---|---|---|
| Catalog # | Size/Concentration | Price |
| Specification | |
|---|---|
| Aliases | SHC; SHCA |
| ProductOrder | S |
| Clone# | 2F7C7 |
| Entrez GenelD | 6464 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | Purified recombinant fragment of human SHC1 (AA: 385-495) expressed in E. Coli. |
| MW | 62.8kDa |
| Shipping Information | This product will ship in a box containing blue ice at a temperature of 4°C. Learn More |
| Species Reactivity | Human, Mouse |
| Application | |
|---|---|
| ELISA | 1/10000 |
| ICC (Immunocytochemistry) | 1/200 - 1/1000 |
| FCM (Flow Cytometry) | 1/200 - 1/400 |
| WB (Western Blot) | 1/500 - 1/2000 |
| Sequence | |
|---|---|
| 385-495 | |
Western Blot
Figure 1: Western blot analysis using SHC1 mAb against human SHC1 (AA: 385-495) recombinant protein. (Expected MW is 37.3 kDa)
Western Blot
Figure 2: Western blot analysis using SHC1 mAb against HEK293 (1) and SHC1 (AA: 385-495)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using SHC1 mouse mAb against NIH/3T3 cell lysate.
Immunofluorescence analysis
Figure 4: Immunofluorescence analysis of A431 cells using SHC1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 5: Flow cytometric analysis of NIH/3T3 cells using SHC1 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);