SHC1 Primary Antibody

Item Information

Catalog #

Size

Price

Description

This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene.

Product Overview

Entrez GenelD

6464

Aliases

SHC; SHCA

Clone#

2F7C7

Host / Isotype

Mouse / IgG1

Species Reactivity

Human, Mouse

Immunogen

Purified recombinant fragment of human SHC1 (AA: 385-495) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1. Am J Physiol Heart Circ Physiol. 2012 Feb 1;302(3):H724-32.
2. Clin Cardiol. 2010 Sep;33(9):548-52.

Product Image

Western Blot

Figure 1: Western blot analysis using SHC1 mAb against human SHC1 (AA: 385-495) recombinant protein. (Expected MW is 37.3 kDa)

Western Blot

Figure 2: Western blot analysis using SHC1 mAb against HEK293 (1) and SHC1 (AA: 385-495)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 3: Western blot analysis using SHC1 mouse mAb against NIH/3T3 cell lysate.

Immunofluorescence analysis

Figure 4: Immunofluorescence analysis of A431 cells using SHC1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric