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SETD7 Primary Antibody
|Aliases||KMT7; SET7; SET9; SET7/9|
|Formulation||Purified antibody in PBS with 0.05% sodium azide|
|Immunogen||Purified recombinant fragment of human SETD7 (AA: 107-366) expressed in E. Coli.|
|Species Reactivity||Human, Rat, Monkey|
|IHC_P (Immunohistochemistry)||1/200 - 1/1000|
|WB (Western Blot)||1/500 - 1/2000|
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using SETD7 mAb against human SETD7 (AA: 107-366) recombinant protein. (Expected MW is 55 kDa)
Figure 3:Western blot analysis using SETD7 mAb against HEK293 (1) and SETD7 (AA: 107-366)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Western blot analysis using SETD7 mouse mAb against MCF-7 (1), Hela (2), A549 (3), COS7 (4), Jurkat (5), and PC-12 (6) cell lysate.
Figure 5:Immunohistochemical analysis of paraffin-embedded Hela tissues using SETD7 mouse mAb with DAB staining.
Histone methyltransferase that specifically monomethylates Lys-4 of histone H3. H3 Lys-4 methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates Lys-189 of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates Lys-372 of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation.
|References (references)||1.PLoS One. 2016 May 16;11(5):e0154939.
2.Sci Rep. 2015 Oct 5;5:14368.