SETD7 Primary Antibody

Item Information
Catalog # Size/Concentration Price
31680 100μg $341.00
Specification
AliasesKMT7; SET7; SET9; SET7/9
Clone#3D6G11
Entrez GeneID80854
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human SETD7 (AA: 107-366) expressed in E. Coli.
MW40.7kDa
Species ReactivityHuman, Rat, Monkey
Application
ELISA1/10000
IHC_P (Immunohistochemistry) 1/200 - 1/1000
WB (Western Blot)1/500 - 1/2000
Sequence
107-366
Catalog
31680
$341.00
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Images

Elisa

Cell Culture Products

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Cell Culture Products

Figure 2:Western blot analysis using SETD7 mAb against human SETD7 (AA: 107-366) recombinant protein. (Expected MW is 55 kDa)

Western Blot

Cell Culture Products

Figure 3:Western blot analysis using SETD7 mAb against HEK293 (1) and SETD7 (AA: 107-366)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Cell Culture Products

Figure 4:Western blot analysis using SETD7 mouse mAb against MCF-7 (1), Hela (2), A549 (3), COS7 (4), Jurkat (5), and PC-12 (6) cell lysate.

Immunohistochemical analysis

Cell Culture Products

Figure 5:Immunohistochemical analysis of paraffin-embedded Hela tissues using SETD7 mouse mAb with DAB staining.

Product Overview
Description

Histone methyltransferase that specifically monomethylates Lys-4 of histone H3. H3 Lys-4 methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates Lys-189 of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates Lys-372 of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation.

References (references)
References (references) 1.PLoS One. 2016 May 16;11(5):e0154939.
2.Sci Rep. 2015 Oct 5;5:14368.

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