SDHB Primary Antibody
Item Information
Catalog #
Size
Price
Description
Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis.
Product Overview
Entrez GenelD
6390
Aliases
IP; SDH; CWS2; PGL4; SDH1; SDH2; SDHIP
Clone#
2B2B1
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human SDHB (AA: 29-280) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4?; -20? for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Endocrine. 2017 Sep;57(3):512-517. 2.J Natl Cancer Inst. 2016 Jan;108(1).
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using SDHB mAb against human SDHB (AA: 29-280) recombinant protein. (Expected MW is 31.7 kDa)
Western Blot
Figure 3:Western blot analysis using SDHB mAb against HEK293 (1) and SDHB (AA: 29-280)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of Hela cells using SDHB mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using SDHB mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.