SDC1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein.
Product Overview
Entrez GenelD
6382
Aliases
SDC; CD138; SYND1; syndecan
Clone#
1A3H4
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Mouse
Immunogen
Purified recombinant fragment of human SDC1 (AA: 28-171) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Am J Clin Pathol. 2012 Mar;137(3):423-8.
2. PLoS One. 2011;6(9):e25252.
2. PLoS One. 2011;6(9):e25252.
Product Image
Western Blot
Figure 1: Western blot analysis using SDC1 mAb against human SDC1 (AA: 28-171) recombinant protein. (Expected MW is 44.4 kDa)
Western Blot
Figure 2: Western blot analysis using SDC1 mAb against HEK293 (1) and SDC1 (AA: 28-171)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using SDC1 mouse mAb against MCF-7 (1), Hela (2), HepG2 (3), T47D (4), SW620 (5), Jurkat (6) and NIH/3T3 (7) cell lysate.
Immunofluorescence analysis
Figure 4: Immunofluorescence analysis of HepG2 cells using SDC1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 5: Flow cytometric analysis of HepG2 cells using SDC1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using SDC1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using SDC1 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.