SDC1 Primary Antibody
|Aliases||SDC; CD138; SYND1; syndecan|
|Formulation||Purified antibody in PBS with 0.05% sodium azide.|
|Immunogen||Purified recombinant fragment of human SDC1 (AA: 28-171) expressed in E. Coli.|
|Shipping Information||This product will ship in a box containing blue ice at a temperature of 4°C. Learn More|
|Species Reactivity||Human, Mouse|
|ICC (Immunocytochemistry)||1/200 - 1/1000|
|IHC_P(Immunohistochemistry)||1/200 - 1/1000|
|FCM (Flow Cytometry)||1/200 - 1/400|
|WB (Western Blot)||1/500 - 1/2000|
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Figure 1: Western blot analysis using SDC1 mAb against human SDC1 (AA: 28-171) recombinant protein. (Expected MW is 44.4 kDa)
Figure 2: Western blot analysis using SDC1 mAb against HEK293 (1) and SDC1 (AA: 28-171)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3: Western blot analysis using SDC1 mouse mAb against MCF-7 (1), Hela (2), HepG2 (3), T47D (4), SW620 (5), Jurkat (6) and NIH/3T3 (7) cell lysate.
Figure 4: Immunofluorescence analysis of HepG2 cells using SDC1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)
Figure 5: Flow cytometric analysis of HepG2 cells using SDC1 mouse mAb (green) and negative control (red).
Figure 6: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using SDC1 mouse mAb with DAB staining.
Figure 7: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using SDC1 mouse mAb with DAB staining.
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
|Description||The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein. |
|References (references)||1. Am J Clin Pathol. 2012 Mar;137(3):423-8. |
2. PLoS One. 2011;6(9):e25252.