Mouse Monoclonal Antibody to SCARB1
Item Information
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Price
Description
The protein encoded by this gene is a plasma membrane receptor for high density lipoprotein cholesterol (HDL). The encoded protein mediates cholesterol transfer to and from HDL. In addition, this protein is a receptor for hepatitis C virus glycoprotein E2. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2019]
Product Overview
Entrez GenelD
949
Aliases
CLA1; SRB1; CLA-1; SR-BI; CD36L1; HDLQTL6
Clone#
2B2A11
Host / Isotype
Mouse / IgG2a
Immunogen
Purified recombinant fragment of human SCARB1 (AA: Extra(33-232)) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Arch Biochem Biophys. 2019 May 15;666:1-7. 2,Cancer Res. 2019 Jul 1;79(13):3320-3331.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using SCARB1 mAb against human SCARB1 (AA: Extra(33-232)) recombinant protein. (Expected MW is 26kDa)
Western Blot
Figure 3:Western blot analysis using SCARB1 mAb against HEK293-6e (1) and human SCARB1 (AA: Extra(33-232))-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using SCARB1 mouse mAb against Hela (1), U937 (2), HePG2 (3), NIH/3T3 (4), and mouse Liver (5) cell lysate.
Flow cytometric analysis
Figure 5:Flow cytometric analysis of Hela cells using SCARB1 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 6:Flow cytometric analysis of U937 cells using SCARB1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using SCARB1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded kidney cancer tissues using SCARB1 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.