RUNX3 Primary Antibody
| Item Information | ||
|---|---|---|
| Catalog # | Size/Concentration | Price |
| Specification | |
|---|---|
| Aliases | AML2; CBFA3; PEBP2aC; FLJ34510; MGC16070 |
| ProductOrder | R |
| Clone# | 2B3 |
| Entrez GenelD | 864 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Host | Mouse |
| Isotype | IgG2b |
| Immunogen | Purified recombinant fragment of human RUNX3 (AA:186-252) expressed in E. Coli. |
| MW | 44.4kDa |
| Shipping Information | This product will ship in a box containing blue ice at a temperature of 4°C. Learn More |
| Species Reactivity | Human, Mouse |
| Application | |
|---|---|
| ELISA | 1/10000 |
| ICC (Immunocytochemistry) | 1/200 - 1/1000 |
| IHC_P(Immunohistochemistry) | 1/200 - 1/1000 |
| FCM (Flow Cytometry) | 1/200 - 1/400 |
| WB (Western Blot) | 1/500 - 1/2000 |
| Sequence | |
|---|---|
| 186-252 | |
Western Blot
Figure 1: Western blot analysis using RUNX3 mAb against human RUNX3 recombinant protein. (Expected MW is 33 kDa)
Western Blot
Figure 2: Western blot analysis using RUNX3 mAb against HEK293 (1) and RUNX3 (AA: 186-252)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4: Flow cytometric analysis of NIH3T3 cells using RUNX3 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using RUNX3 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);