RUNX3 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesAML2; CBFA3; PEBP2aC; FLJ34510; MGC16070
ProductOrderR
Clone#2B3
Entrez GenelD864
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG2b
ImmunogenPurified recombinant fragment of human RUNX3 (AA:186-252) expressed in E. Coli.
MW44.4kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman, Mouse
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
186-252
Catalog#: 30408
Inquire
Contact us to order
Images
Western Blot
Figure 1: Western blot analysis using RUNX3 mAb against human RUNX3 recombinant protein. (Expected MW is 33 kDa)
Figure 1: Western blot analysis using RUNX3 mAb against human RUNX3 recombinant protein. (Expected MW is 33 kDa)
Western Blot
Figure 2: Western blot analysis using RUNX3 mAb against HEK293 (1) and RUNX3 (AA: 186-252)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using RUNX3 mAb against HEK293 (1) and RUNX3 (AA: 186-252)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4: Flow cytometric analysis of NIH3T3 cells using RUNX3 mouse mAb (green) and negative control (red).
Figure 4: Flow cytometric analysis of NIH3T3 cells using RUNX3 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using RUNX3 mouse mAb with DAB staining.
Figure 5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using RUNX3 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Multiple transcript variants encoding different isoforms have been found for this gene.

References (references)
References (references)1.J Cancer Res Clin Oncol. 2011 Dec;137(12):1823-30. 2.Oncogene. 2012 Jan 26;31(4):527-34.