RUNX1 Primary Antibody

Item Information
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Description

Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The protein encoded by this gene represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Three transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq) Tissue specificity: Expressed in all tissues examined except brain and heart. Highest levels in thymus, bone marrow and peripheral blood.

Product Overview
Entrez GenelD
861
Aliases
AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; AML1-EVI-1; RUNX1
Clone#
5A1
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Synthesized peptide of human RUNX1.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. J Cell Physiol. 2009 Feb;218(2):343-9.
2. J Radiat Res (Tokyo). 2008 Sep;49(5):549-55.
Product Image
Western Blot
Figure 1: Western blot analysis using RUNX1 mouse mAb against Jurkat cell lysate.
Figure 1: Western blot analysis using RUNX1 mouse mAb against Jurkat cell lysate.
Immunofluorescence analysis
Figure 2: Immunofluorescence analysis of Hela cells using RUNX1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 2: Immunofluorescence analysis of Hela cells using RUNX1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Elisa
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
For Research Use Only. Not for use in diagnostic procedures.