RSK2 Primary Antibody
| Item Information | ||
|---|---|---|
| Catalog # | Size/Concentration | Price |
| Specification | |
|---|---|
| Aliases | RPS6KA3; CLS; RSK; HU-3; RSK2; MRX19; ISPK-1; p90-RSK2; pp90RSK2; MAPKAPK1B; S6K-alpha3 |
| ProductOrder | R |
| Clone# | 4E10 |
| Entrez GenelD | 6197 |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | Purified recombinant fragment of human RSK2 expressed in E. Coli. |
| MW | 80kDa |
| Shipping Information | This product will ship in a box containing blue ice at a temperature of 4°C. Learn More |
| Species Reactivity | Human |
| Application | |
|---|---|
| ELISA | 1/10000 |
| ICC (Immunocytochemistry) | 1/200 - 1/1000 |
| FCM (Flow Cytometry) | 1/200 - 1/400 |
| WB (Western Blot) | 1/500 - 1/2000 |
| Sequence | |
|---|---|
| 1-212 | |
Western Blot
Figure 1: Western blot analysis using RSK2 mAb against human RSK2 (AA: 1-212) recombinant protein. (Expected MW is 49.7 kDa)
Western Blot
Figure 2: Western blot analysis using RSK2 mouse mAb against Hela (1), MCF-7 (2), and HepG2 (3) cell lysate.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of HepG2 cells using RSK2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric
Figure 4: Flow cytometric analysis of HepG2 cells using RSK2 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);