RSK2 Primary Antibody

Item Information

Catalog #

Size

Price

Description

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS).

Product Overview

Entrez GenelD

6197

Aliases

RPS6KA3; CLS; RSK; HU-3; RSK2; MRX19; ISPK-1; p90-RSK2; pp90RSK2; MAPKAPK1B; S6K-alpha3

Clone#

4E10

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human RSK2 expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1. Mol Cell. 2009 Jan 16;33(1):109-16.
2. Cancer Res. 2009 May 15;69(10):4398-406.

Product Image

Western Blot

Figure 1: Western blot analysis using RSK2 mAb against human RSK2 (AA: 1-212) recombinant protein. (Expected MW is 49.7 kDa)

Western Blot

Figure 2: Western blot analysis using RSK2 mouse mAb against Hela (1), MCF-7 (2), and HepG2 (3) cell lysate.

Immunofluorescence analysis

Figure 3: Immunofluorescence analysis of HepG2 cells using RSK2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow cytometric