RPS6KB1 Primary Antibody
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Description
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized.
Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
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Western Blot

Figure 1: Western blot analysis using RPS6KB1 mAb against human RPS6KB1 (AA: 295-524) recombinant protein. (Expected MW is 59 kDa)
Immunohistochemical analysis

Figure 2: Immunohistochemical analysis of paraffin-embedded breast cancer tissues using RPS6KB1 mouse mAb with DAB staining.
Immunohistochemical analysis

Figure 3: Immunohistochemical analysis of paraffin-embedded muscle tissues using RPS6KB1 mouse mAb with DAB staining.
Flow cytometric

Figure 4: Flow cytometric analysis of Jurkat cells using RPS6KB1 mouse mAb (green) and negative control (red).
Elisa

Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.