RPS6KB1 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesS6K; PS6K; S6K1; STK14A; p70-S6K; p70-alpha; p70(S6K)-alpha
ProductOrderR
Clone#5G9
Entrez GenelD6198
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human RPS6KB1 expressed in E. Coli.
MW59kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
295-524
Catalog#: 30319
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Images
Western Blot
Figure 1: Western blot analysis using RPS6KB1 mAb against human RPS6KB1 (AA: 295-524) recombinant protein. (Expected MW is 59 kDa)
Figure 1: Western blot analysis using RPS6KB1 mAb against human RPS6KB1 (AA: 295-524) recombinant protein. (Expected MW is 59 kDa)
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded breast cancer tissues using RPS6KB1 mouse mAb with DAB staining.
Figure 2: Immunohistochemical analysis of paraffin-embedded breast cancer tissues using RPS6KB1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded muscle tissues using RPS6KB1 mouse mAb with DAB staining.
Figure 3: Immunohistochemical analysis of paraffin-embedded muscle tissues using RPS6KB1 mouse mAb with DAB staining.
Flow cytometric
Figure 4: Flow cytometric analysis of Jurkat cells using RPS6KB1 mouse mAb (green) and negative control (red).
Figure 4: Flow cytometric analysis of Jurkat cells using RPS6KB1 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized.

References (references)
References (references)1. J Biol Chem. 2010 Jan 1;285(1):30-42.
2. Cell Mol Life Sci. 2009 Apr;66(8):1457-66.