RPS6KA2 Primary Antibody
| Item Information | ||
|---|---|---|
| Catalog # | Size/Concentration | Price |
| Specification | |
|---|---|
| Aliases | RSK; HU-2; RSK3; p90-RSK3; pp90RSK3; MAPKAPK1C; S6K-alpha; S6K-alpha2 |
| ProductOrder | R |
| Clone# | 3C4C8 |
| Entrez GenelD | 6196 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | Purified recombinant fragment of human RPS6KA2 (AA: 415-734) expressed in E. Coli. |
| MW | 83.2kDa |
| Shipping Information | This product will ship in a box containing blue ice at a temperature of 4°C. Learn More |
| Species Reactivity | Human, Mouse |
| Application | |
|---|---|
| ELISA | 1/10000 |
| ICC (Immunocytochemistry) | 1/200 - 1/1000 |
| IHC_P(Immunohistochemistry) | 1/200 - 1/1000 |
| FCM (Flow Cytometry) | 1/200 - 1/400 |
| WB (Western Blot) | 1/500 - 1/2000 |
| Sequence | |
|---|---|
| 415-734 | |
Western Blot
Figure 1: Western blot analysis using RPS6KA2 mAb against human RPS6KA2 (AA: 415-734) recombinant protein. (Expected MW is 62 kDa)
Western Blot
Figure 2: Western blot analysis using RPS6KA2 mAb against HEK293 (1) and RPS6KA2 (AA: 415-734)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using RPS6KA2 mouse mAb against Hela (1), A431 (2), HEK293 (3), Jurkat (4), HepG2 (5), MCF-7 (6), NIH/3T3 (7) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of A431 cells using RPS6KA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 5: Flow cytometric analysis of Hela cells using RPS6KA2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using RPS6KA2 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using RPS6KA2 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);