RPS6KA2 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesRSK; HU-2; RSK3; p90-RSK3; pp90RSK3; MAPKAPK1C; S6K-alpha; S6K-alpha2
ProductOrderR
Clone#3C4C8
Entrez GenelD6196
FormulationPurified antibody in PBS with 0.05% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human RPS6KA2 (AA: 415-734) expressed in E. Coli.
MW83.2kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman, Mouse
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
415-734
Catalog#: 30694
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Images
Western Blot
Figure 1: Western blot analysis using RPS6KA2 mAb against human RPS6KA2 (AA: 415-734) recombinant protein. (Expected MW is 62 kDa)
Figure 1: Western blot analysis using RPS6KA2 mAb against human RPS6KA2 (AA: 415-734) recombinant protein. (Expected MW is 62 kDa)
Western Blot
Figure 2: Western blot analysis using RPS6KA2 mAb against HEK293 (1) and RPS6KA2 (AA: 415-734)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using RPS6KA2 mAb against HEK293 (1) and RPS6KA2 (AA: 415-734)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using RPS6KA2 mouse mAb against Hela (1), A431 (2), HEK293 (3), Jurkat (4), HepG2 (5), MCF-7 (6), NIH/3T3 (7) cell lysate.
Figure 3: Western blot analysis using RPS6KA2 mouse mAb against Hela (1), A431 (2), HEK293 (3), Jurkat (4), HepG2 (5), MCF-7 (6), NIH/3T3 (7) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of A431 cells using RPS6KA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4:Immunofluorescence analysis of A431 cells using RPS6KA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 5: Flow cytometric analysis of Hela cells using RPS6KA2 mouse mAb (green) and negative control (red).
Figure 5: Flow cytometric analysis of Hela cells using RPS6KA2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using RPS6KA2 mouse mAb with DAB staining.
Figure 6: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using RPS6KA2 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using RPS6KA2 mouse mAb with DAB staining.
Figure 7: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using RPS6KA2 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

References (references)
References (references)1. Oncogene. 2007 Feb 1;26(5):683-700.
2. Exp Mol Med. 2003 Oct 31;35(5):365-70.