RPA1 Primary Antibody

Item Information

Catalog #

Size

Price

Description

"This gene Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing"

Product Overview

Entrez GenelD

6117

Aliases

HSSB; RF-A; RP-A; REPA1; RPA70; MST075

Clone#

4C4

Host / Isotype

Mouse / IgG1

Species Reactivity

Human, Monkey

Immunogen

Purified recombinant fragment of human RPA1 expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

Mol Cell. 2009 Oct 23;36(2):193-206.
J Biol Chem. 2009 Dec 11;284(50):34682-91.

Product Image

Western Blot

Figure 1: Western blot analysis using RPA1 mAb against human RPA1 (AA: 308-513) recombinant protein. (Expected MW is 48.3 kDa)

Western Blot

Figure 2: Western blot analysis using RPA1 mouse mAb against HeLa (1), MCF-7 (2), K562(3), A431(4), and COS-7 (6) cell lysate.

Immunohistochemical analysis

Figure 3: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using RPA1 mouse mAb with DAB staining.

Immunohistochemical analysis

Figure 4: Immunohistochemical analysis of paraffin-embedded prostate tissues using RPA1 mouse mAb with DAB staining.

Immunofluorescence analysis

Figure 5: Immunofluorescence analysis of HeLa cells using RPA1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow cytometric