RND3 Primary Antibody
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Description
This gene encodes a protein which is a member of the small GTPase protein superfamily. The encoded protein binds only GTP but has no GTPase activity, and appears to act as a negative regulator of cytoskeletal organization leading to loss of adhesion. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using RND3 mAb against human RND3 (AA: 104-241) recombinant protein. (Expected MW is 41.3 kDa)
Western Blot
Figure 3:Western blot analysis using RND3 mAb against HEK293 (1) and RND3 (AA: 104-241)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of Hela cells using RND3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 5:Flow cytometric analysis of Hela cells using RND3 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using RND3 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.