Ring1 Primary Antibody
| Item Information | ||
|---|---|---|
| Catalog # | Size/Concentration | Price |
| Specification | |
|---|---|
| Aliases | RNF1; RING1A |
| ProductOrder | R |
| Clone# | 8E8A2 |
| Entrez GenelD | 6015 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | Purified recombinant fragment of human Ring1 (AA: 79-263) expressed in E. Coli. |
| MW | 42.4kDa |
| Shipping Information | This product will ship in a box containing blue ice at a temperature of 4°C. Learn More |
| Species Reactivity | Human |
| Application | |
|---|---|
| ELISA | 1/10000 |
| ICC (Immunocytochemistry) | 1/200 - 1/1000 |
| WB (Western Blot) | 1/500 - 1/2000 |
| Sequence | |
|---|---|
| 79-263 | |
Western Blot
Figure 1: Western blot analysis using Ring1 mAb against human Ring1 recombinant protein. (Expected MW is 44.6 kDa)
Western Blot
Figure 2: Western blot analysis using Ring1 mAb against HEK293 (1) and Ring1 (AA: 79-263)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of A431 cells using Ring1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of Hela cells using Ring1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);