RICTOR Primary Antibody

Item Information

Catalog #

Size

Price

Description

Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian TOR (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals . mTOR is a large protein kinase with two different complexes. One complex contains mTOR, G

Product Overview

Entrez GenelD

253260

Aliases

PIA; mAVO3; KIAA1999; MGC39830; DKFZp686B11164; RICTOR

Clone#

7B3

Host / Isotype

Mouse / IgG1

Species Reactivity

Human, Monkey, Mouse

Immunogen

Purified recombinant fragment of human RICTOR expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1. Genes Dev. 2006 Oct 15;20(20):2820-32.
2. Biochem Biophys Res Commun. 2008 Aug 8;372(4):578-83.

Product Image

Western Blot

Figure 1: Western blot analysis using RICTOR mouse mAb against Hela (1), PANC-1 (2), MOLT4 (3), HepG2 (4) and HEK293 (5) cell lysate.

Immunofluorescence analysis

Figure 2: Immunofluorescence analysis of NIH/3T3 cells using RICTOR mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemical analysis

Figure 3: Immunohistochemical analysis of paraffin-embedded thyroid gland tissues (left) and human breast carcinoma (right) using RICTOR mouse mAb with DAB staining.

Flow cytometric