RF1 Primary Antibody
This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 6, 7, and X.
Purified antibody in PBS with 0.05% sodium azide
4°C; -20°C for long term storage
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using RF1 mAb against human RF1 (AA: 288-437) recombinant protein. (Expected MW is 43 kDa)
Figure 3:Western blot analysis using RF1 mAb against HEK293 (1) and RF1 (AA: 288-437)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 5:Flow cytometric analysis of Hela cells using RF1 mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of HepG2 cells using RF1 mouse mAb (green) and negative control (red).
Figure 6:Western blot analysis using *** mouse mAb against MCF-7 (1), T47D (2), MOLT4 (3), and Raji (4) cell lysate.
For Research Use Only. Not for use in diagnostic procedures.