RELB Primary Antibody
NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric RelB-p50 and RelB-p52 complexes are transcriptional activators. RELB neither associates with DNA nor with RELA/p65 or REL. Stimulates promoter activity in the presence of NFKB2/p49. As a member of the NUPR1/RELB/IER3 survival pathway, may provide pancreatic ductal adenocarcinoma with remarkable resistance to cell stress, such as starvation or gemcitabine treatment
2. Cancer Res. 2009 Apr 15;69(8):3267-71.
![Figure 1: Western blot analysis using RELB mAb against human RELB (AA: 273-443) recombinant protein. (Expected MW is 45.5 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/30663rwb.jpg?v=1666700630)
![Figure 2: Western blot analysis using RELB mAb against HEK293 (1) and RELB (AA: 273-443)-hIgGFc transfected HEK293 (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/30663twb.jpg?v=1666700630)
![Figure 3: Immunofluorescence analysis of MCF-7 cells using RELB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/30663icc.jpg?v=1666700630)
![Figure 4: Flow cytometric analysis of MCF-7 cells using RELB mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/30663fcm.jpg?v=1666700630)
![Figure 5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using RELB mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/30663ihc-1.jpg?v=1666700630)
![Figure 6: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using RELB mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/30663ihc-2.jpg?v=1666700630)
![Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/30663ea.jpg?v=1666700630)