RB1 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesRB; pRb; OSRC; pp110; p105-Rb
ProductOrderR
Clone#2A11D11
Entrez GenelD5925
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human RB1 (AA: 2106-2784) expressed in E. Coli.
MW106.2kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
2106-2784
Catalog#: 30584
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Images
Western Blot
Figure 1: Western blot analysis using RB1 mAb against human RB1 (AA: 2106-2784) recombinant protein. (Expected MW is 38.8 kDa)
Figure 1: Western blot analysis using RB1 mAb against human RB1 (AA: 2106-2784) recombinant protein. (Expected MW is 38.8 kDa)
Western Blot
Figure 2: Western blot analysis using RB1 mouse mAb against Jurkat (1) and A431 (2) cell lysate.
Figure 2: Western blot analysis using RB1 mouse mAb against Jurkat (1) and A431 (2) cell lysate.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of NIH/3T3 cells using RB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 3: Immunofluorescence analysis of NIH/3T3 cells using RB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 4: Flow cytometric analysis of Jurkat cells using RB1 mouse mAb (green) and negative control (red).
Figure 4: Flow cytometric analysis of Jurkat cells using RB1 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
DescriptionThe protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.



References (references)
References (references)1. Cancer Genet. 2011 Jun;204(6):316-22.
2. J Neuropathol Exp Neurol. 2012 Jan;71(1):83-9.